Effect of in feed administration of different butyrate formulations on Salmonella Enteritidis colonization and cecal microbiota in broilers

Table 7 Relative abundances of bacterial phyla, families and genera in the microbial community in ceca of broilers

Phylum	Family	Genus	Dietary treatment
			Control		Wax		Fat-protected butyrate
			Mean	SD	Mean	SD	Mean	SD
Firmicutes			91.63^a	6.10	93.94^a	2.92	94.57^a	2.73
	Lachnospiraceae		52.75^a	12.17	62.48^b	6.13	50.68^a	11.56
		Blautia	12.12^a	4.26	16.44^b	4.87	12.96^ab	4.51
		Other/uncultured	29.80^a	10.22	37.83^b	7.05	29.72^ab	11.06
	Ruminococcaceae		18.38^ab	9.15	13.85^a	4.85	24.15^b	9.19
		Subdoligranulum	4.56^a	7.44	0.31^b	0.41	5.85^ab	10.65
		Anaerotruncus	2.55^a	1.66	2.23^a	1.51	2.98^a	1.65
		Other/uncultured	11.03^a	4.18	11.19^a	4.04	14.69^a	5.16
	Lactobacillaceae		10.22^a	8.19	3.82^b	3.86	7.54^ab	6.14
		Lactobacillus	10.22^a	8.19	3.82^b	3.86	7.54^ab	6.14
	Streptococcaceae		7.91^a	4.54	8.34^a	4.95	7.96^a	3.00
	VadinBB60		1.14^a	1.02	3.62^b	2.33	2.44^ab	2.77
	Defluviitaleaceae		0.15^a	0.05	0.30^b	0.16	0.03^b	0.14
	Peptoniphilaceae		0.05^a	0.04	0.01^b	0.02	0.00^b	0.01
	Lachnospiraceae + Ruminococcaceae		71.13^a	8.11	76.33^a	6.84	74.84^a	7.19
Proteobacteria			3.16^a	5.28	0.56^b	0.45	0.91^b	1.32
	Enterobacteriaceae		1.80^a	1.21	0.54^b	0.44	0.29^b	1.32
		Escherichia/Shigella	1.76^a	1.17	0.54^b	0.44	0.88^b	1.33
Actinobacteria			91.64^a	6.10	93.94^a	2.92	94.58^a	2.73
Other			4.69^a	3.58	5.03^a	2.87	4.11^a	2.20

At the age of 21 days after 4 days of Salmonella infection relative abundances in cecal content were determined with 16S rRNA V3–V4 amplicon sequencing. The broilers were fed a diet either or not supplemented with a butyrate containing feed additive in a concentration of 3 g/kg of sodium butyrate. Each dietary treatment consisted of 2 pens of 20 broilers.
Significant differences for relative abundances of bacterial phyla, families and genera among dietary treatments are indicated with different letters per row (a, b).

ISSN: 1297-9716