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Table 3 Infectivity of heat and protease treated whole brain homogenates in the host rodent species

From: Stability of BSE infectivity towards heat treatment even after proteolytic removal of prion protein

Infection model Treatment
Detergent, no heat Detergent, heat Detergent, heat, KE Detergent, heat, PK NEG Cntrlsa
263 K in hamsters 6/6 (ID50 = 6.0–6.5) 0/6 (ID50 < 3.5) 0/6 (ID50 < 3.5) 0/6 (ID50 < 3.5) 0/12 (ID50 < 3.5)
117 ± 7 d  > 325 d  > 325 d  > 325 d  > 284 d
PG127 scrapie in tg338 mice 6/6 (ID50 = 5,8–6,3) 0/6 (ID50 < 2,5 ID) 0/6 (ID50 < 2,5 ID) 0/6 (ID50 < 2,5 ID) 0/12 (ID50 < 2.5)
82 ± 4 d  > 200 d  > 200 d  > 200 d  > 200 d
109ICWD1 in Bv109I bank voles 6/6b (ID50 = 8.4) 1/6 (ID50 = 3.4) 0/6 (ID =  < 3) 0/6 (ID =  < 3) 0/12 (ID50 < 3)
44 ± 4 d 318 d  > 450 d  > 450 d  > 450 d
  1. Inocula tested at 1% tissue concentration, except where indicated. Titres were based on survival times and deduced for hamster 263 K scrapie as in [1], for sheep PG127 scrapie from end-point titration curve in [43], and for CWD1109I from end-point titration curve in [42].
  2. a12 animals, three in each of the four treatments.
  3. bunheated material tested with 0.1% inoculum; other treatments with bank voles were in addition to 1% also checked at 0.1 and 0.01% tissue concentrations and all with no attacks as result.
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Veterinary Research

ISSN: 1297-9716

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